By using the ProPHECY™ method you will:
✓ Reduce the effort, time and costs involved in optimizing one or several target protein or peptide properties
✓ Reach your goals faster and at lower cost compared to random screening
✓ Discover hidden information in large and complex data sets
✓ Predict the effect of a single amino acid replacement from data containing complex replacement patterns
✓ Achieve detailed quantitative information on the influence of each amino acid replacement
✓ Perform parallel optimization of several parameters
✓ Understand sequence-activity-relationships for your target protein or peptide
✓ Use the gained information to rationally predict and design new optimized variants
Download the ProPHECY flyer for more information!
Examples of the use of ProPHECY™ can also be found in the publications below:
(1) Pasupuleti M et al., (2008). Biochemistry, 47, 9057-9070.
(2) Kassetty, G. et al., (2011). J Innate Immun, 3, 471-482.
About the method
It is often desirable to modify a biomolecule (peptide, polypeptide, protein, antibody etc.) to optimize specific properties in a rational way. For example:
✓ increase its biological or catalytic activity
✓ decrease its antigenicity
✓ enhance its cell penetrating capabilities
✓ protease resistance,
✓ thermal stability,
Our proprietary and proven ProPHECY™ in silico protein optimization technology offers a quick and less costly approach.
By modifying and optimizing the amino acid sequence of a protein one could explore the structure-activity relationships (SAR) and using them control protein properties and eventually modify them in a desired way. ProPHECY™ is reminiscent of QSAR (Quantitative Structure-Activity Relationship) methodology used for decades in the optimization of organic small molecules. It uses a novel method by which experimentally determined properties of a protein can be utilized for the prediction of amino acid replacements required to achieve controlled modifications. This will greatly reduce effort, time and costs required in a protein optimization project. You are welcome to contact us for further discussion of your project.