My Image

Structural Biology Services: Protein Structure Determination and Drug Discovery

Structural biology services at SARomics Biostructures are built around state of the art high-throughput gene-to-structure platform, with high-throughput protein expression, purification, protein crystallization and structure determination by X-ray crystallography and NMR spectroscopy. With proven expertise in protein structure analysis, we add to our customers’ projects clear insights into the mode of binding and the mechanisms of interactions in ligand-protein and antigen-antibody complexes. In addition, our structure-based drug discovery services are based on fragment library screening using our proprietary weak affinity chromatography technology (WAC™ ), hit expansion and optimization guided by protein-ligand complex structures. The following pages describe details of the technology platform and are design to help our customers in the preparation for the experiments:

Protein X-ray crystallography (fee-for-service crystallography)
NMR spectroscopy
Computational chemistry & drug discovery

SARomics’ structural biology platform include all major techniques required for successful protein structure determination. Following cloning, expression and purification of the protein (gene-to-structure services), and before the start of a crystallography or NMR spectroscopy project, we perform a thorough biophysical characterization of the protein in solution - both for successful crystallization and for NMR studies we need monodisperse solutions, which do not contain any aggregated or denatured material. The protein also needs to be stable within a certain range of pH, ionic strength and temperature (these values will be varied when crystallization screens are run). It should always be kept in mind that a proper characterization of the protein solution is crucial for the success of a structure determination project!
Several biophysical techniques are routinely used at SARomics Biostructures for protein characterization - NMR spectroscopy, dynamic light scattering (DLS), CD spectroscopy and
differential scanning fluorimetry (DSF, similar to thermal-shift assay). Please see the following pages for method-specific experimental details.