SARomics Biostructures structural biology services include protein expression and purification, crystallization and structure determination by X-ray crystallography and NMR spectroscopy. Our drug discovery services also include fragment library screening, hit expansion and optimization and protein-ligand co-crystallization. The following pages describe details of the technology platform and are design to help our customers in the preparation for the desired experiments:
Our structural biology services include all steps required for successful protein structure determination. Following cloning, expression and purification of the protein (gene-to-structure services), and before the start of a crystallography or NMR spectroscopy project, we perform a thorough characterization of the protein in solution - both for successful crystallization and for NMR studies we need a monodisperse solution, which does not contain any aggregated or denatured material. The protein also needs to be stable within a certain range of pH, ionic strength and temperature (these values will be varied when crystallization screens are run). It should always be kept in mind that a proper characterization of the protein solution is crucial for the success of a structure determination project!
Several biophysical techniques are routinely used at SARomics Biostructures for protein characterization - NMR spectroscopy, dynamic light scattering (DLS), CD spectroscopy and differential scanning fluorimetry (DSF, similar to thermal-shift assay).